Part:BBa_K1110001

pGAPzalphaC+standardizing linker

The plasmid pGAPzalphaC was standardized according to iGEM standards using a linker piece

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3126
  Illegal SpeI site found at 2
  Illegal PstI site found at 24
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3126
  Illegal SpeI site found at 2
  Illegal PstI site found at 24
  Illegal NotI site found at 13
  Illegal NotI site found at 3137
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3126
  Illegal BglII site found at 2362
  Illegal BamHI site found at 442
  Illegal XhoI site found at 3097
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3126
  Illegal SpeI site found at 2
  Illegal PstI site found at 24
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3126
  Illegal SpeI site found at 2
  Illegal PstI site found at 24
  Illegal NgoMIV site found at 1199
  Illegal NgoMIV site found at 1260
  Illegal AgeI site found at 161
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 425
  Illegal BsaI.rc site found at 624
  Illegal BsaI.rc site found at 1621
  Illegal SapI site found at 2605